ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers

Abstract Background Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) abiotic (phenolic compounds). In order to clarify proteomic differences Fusarium moniliforme under action phloridzin, explore potential mechanism F. as pathogen ARD, role spp. in ARD was further clarified. Methods this paper, quantitative proteomics method iTRAQ analysis technology used analyze before after phloridzin treatment. The differentially expressed protein validated qRT-PCR analysis. Results A total 4535 proteins were detected, 293 found with more than 1.2 times ( P < 0.05) differences. In-depth data revealed that 59 1.5 differences, most consistent result qRT-PCR. Differentially influenced a variety cellular processes, particularly metabolic processes. Among these pathways, 8 significantly enriched KEGG pathways identified at least 2 affiliated different abundance conidia mycelium. Functional pathway indicated up-regulated mainly distributed amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis phagosome pathways. Conclusions This study first perform investigation labeling LC-MS/MS identify conditions. results confirmed presented unique profile adaptive mechanisms species environments. deepened our understanding proteome response inducers provide basis for exploration improving efficiency fungi biocontrol agents control ARD.